An atlas of the clinical microbiology of infectious - download pdf or read online

By Edward J. Bottone

ISBN-10: 0203491610

ISBN-13: 9780203491614

content material: v. 1. Bacterial brokers --
v. 2. Viral, fungal, and parasitic agents.

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Extra resources for An atlas of the clinical microbiology of infectious diseases. / Volume 1, Bacterial agents

Example text

Tuberculosis Figure 87 Mycobacterium tuberculosis Apple­green to yellow fluorescing bacilli in sputum smear stained with rhodamine—auramine.  Granules represent individual colonies and are comprised of tightly interwoven bacilli in cord formation Page 33 Morphology The bacilli are either straight or curved to elongated rods, often occurring singly or banded together (cording) because of their lipid­rich hydrophobic surface.  tuberculosis requires enriched media for growth, as provided by Lowenstein­Jensen (egg­based) and Middlebrook 7H10 and 7H11 (serum­based) media.

Culture of in­use shaving cream was negative Figure 131 Mycobacterium chelonei Smooth colonies growing on 5% sheep blood agar 48 h after touch inoculation of agar with tissue fragments scraped from leg lesion Figure 132 Mycobacterium chelonei Dry, rough, crinkled colonies recovered from broviac catheter segment rolled over agar surface.  chelonei is negative for this characteristic.  Its natural habitat is unknown.  leprae is the causative agent of leprosy (Hansen’s disease), a chronic disease of the skin, peripheral nerves, and mucus membranes, leading to loss of sensation (anesthesia) and hypopigmentation.

Shigellae are enteroinvasive and can cause necrosis of the colonic epithelium.  Colonies of Salmonella typhi (typhoid bacillus) may appear green without dark centers Page 53 Figure 181 Salmonella species Giemsa stain of bone marrow aspirate of child with AIDS showing intracellular bacilli in macrophage.  typhimurium.  Salmonellae are lactose­negative and most serovars produce hydrogen sulfide.  This species produces a number of proteases and an extracellular deoxyribonuclease.  Y.  enterocolitica which produces dark red colonies on this medium.

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An atlas of the clinical microbiology of infectious diseases. / Volume 1, Bacterial agents by Edward J. Bottone


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