Advances in Biochemical Engineering, Volume 009: Microbial by T.K. Ghose ,A. Fiechter, N.Blakebrough PDF

By T.K. Ghose ,A. Fiechter, N.Blakebrough

ISBN-10: 0387086064

ISBN-13: 9780387086064

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However, it was also observed that a significant fraction of the cells were free from large oil drops. About 30% of the cells in the culture broth precipitated during centrifugation. This suggests that more than one-half of the cells may be attached to large oil drops. e. direct contact of cells with large drops and utilization of submicron droplets, may be important for hydrocarbon uptake by microorganisms. Nakahara et al. [33] also showed that the interfacial tension between oil and water decreased sharply as cultivation proceeds and as the size of the oil drop decreases.

Therefore, Monod's model is not valid for the growth rate of C. intermedia on a n-tetradecane medium. C. intermedia has a strong affinity for hydrocarbon and is considered to contain a hydrocarbon pool in and/or on the cell, as inferred from Fig. 13. It is, therefore, coneluded that the growth rate of C. interrnedia on hydrocarbon is a function of the hydrocarbon pool. The hydrocarbon pool is related to the specific concentration of hydrocarbon per unit cell mass in the medium by the term S • X -1.

2. Affinity of Microorganisms for Liquid Hydrocarbon . . . . . . . . . . . 1 Adherent Characteristics . . . . . . . . . . . . . . . . . . 2 Adhesive Force . . . . . . . . . . . . . . . . . . . . 3. Pathway for Liquid Hydrocarbon Uptake . . . . . . . . . . . . . . 4. Hydrocarbon Pool . . . . . . . . . . . . . . . . . . . . . 5. Growth Kinetics of Microorganisms with Low Affinity for Liquid Hydrocarbon .

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Advances in Biochemical Engineering, Volume 009: Microbial Processes by T.K. Ghose ,A. Fiechter, N.Blakebrough


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